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adjusting themselves to regain ability to infect mutated bacteria (Weitz et al. 2005). Another major drawback of phage technology is the dif culty of getting phages to kill mesophilic and psychrotrophic microorganisms in a food product stored at refrigeration temperatures. Phage activity requires actively growing and dividing hosts, which for many mesophiles generally does not occur in a chilled stored product. Although psychotrophs can grow at chill temperatures, their growth rate is slow. For phage to be effective against spoilers their activity must be certain at low storage temperatures. Another issue is that large initial bacterial populations (3 to 5 log10 CFU/g) are needed for phage activity (Greer 2005; Hudson et al. 2005). Since phage attack on bacteria initiates upon contact, the probability of this encounter increases if populations of both counterparts are large. If spoilage bacterial numbers are low on a product due to the effectiveness of other control measures, then phage treatment may not be valuable. However, in the case of refrigerated meat spoilage, where generally large population numbers are present, phage technology may have merit. The nature of the environment can play a signi cant role in phage attack, with viscous product physically reducing the possibility of phage-host interaction. Other drawbacks include the possibility of phage-mediated virulence gene transfer between bacteria. Lysogenic conversion of lytic phage to temperate phage could result in host protection from other phages. As a last argument, the average consumer associates viruses with disease, and their perception of adding viruses to foods has yet to be investigated. To summarize, the biggest challenge in utilizing phage technology in modern meat spoilage control might be high phage speci city, which makes it dif cult to achieve the desired results of shelf life extension for naturally contaminated meat products. Genetic manipulation of available phages rdlc pdf 417 PDF417 Barcode Creating Library for RDLC Reports | Generate ...
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